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KMID : 0363320050260010107
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Journal of Korean Oriental Internal Medicine
2005 Volume.26 No. 1 p.107 ~ p.118
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Peroxynitrite Scavenging Mechanism of Ojawhan
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Kim Hyung-Joon
Jung Ji-Chun
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Abstract
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Objectives : Peroxynitrite (ONOO-), fonned from the reaction of O_2- and NO, is a cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer’s disease, rheumatoid arthritis, cancer and atherosclerosis. Due to the lack of endogenous enzymes to thwart ONOO- activation, developing a specific ONOO- scavenger is remarkably important. The aim of this study was to investigate scavenging activities of ONOO- and its precursors, NO and O_2- and its scavenging mechanism of Ojawhan.
Methods : To investigate scavenging activities of ONOO-, NO, O_2- and its scavenging mechanism using fluorescent probes, DCFDA, DAF-2 and DHR 123. The ONOO- scavenging activity on Ojawhan was assayed by measuring oxidized dihydrorhodamine 123 (DHR 123) by fluorometry. Oxidative stress was induced by strong oxidants t-butyl hydroperoxide (t-BHP). Endothelial cell (YPEN-1) was used for detection of intracellular oxidative stress.
Results : Ojawhan markedly scavenged authentic ONOO-, O_2- and NO. It also inhibited ONOO- induced by O_2- and NO which are derived from SIN-1. Furthennore, {underline{Ojawhan}} blocked lipopolysaccharide (LPS)-induced ONOO-, O_2- and NO generation utilizing kidney homogenates of LPS-injected mouse and inhibited t-BHP-induced ROS and ONOO- in endothelial cell culture system.
Conclusions : These results suggest that Ojawhan be developed as an effective ONOO- scavenger for the prevention of ONOO- involved diseases and age-related diseases.
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KEYWORD
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Peroxynitrite, Scavenging Mechanism, Ojawhan, t-butylhydroperoxide(t-BHP)
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